Journal: Frontiers in Neuroscience

Article Title: Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality

doi: 10.3389/fnins.2023.1198154

Figure Lengend Snippet: Result summary of tested antibodies in our optimized protocol.

Article Snippet: PSD95 , Excitatory postsynapse , ms , StressMarq , SMC-122D , AB_2300386 , 1:500; 2 μg/mL , No.

Techniques: Marker, Concentration Assay

Journal: IBRO Neuroscience Reports

Article Title: IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway

doi: 10.1016/j.ibneur.2024.07.008

Figure Lengend Snippet: Microglial cells were activated and transformed to the M2 phenotype after treatment with IL-33. (a) Western blot images and the quantification of CD206 and CD40 levels after treatment with IL-33 or NMOD serum. (b) BV2 cells were labeled with Iba-1 and CD68 for immunofluorescence. (c) Expression levels of PSD95 were observed by immunofluorescence after treatment with IL-33 or NMOSD serum. One-way analysis of variance was used for statistical analyses. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: For protein immunoreactivity in cells, the primary cortical neurons or BV2 cells were fixed with 4 % paraformaldehyde for 30 min at RT and treated with 0.5 % Triton for 10 min. After being washed with PBS, the cells were blocked with 5 % bovine serum albumin (BSA) for 1 h and then incubated with a rabbit anti-PSD95 antibody (1:100 dilution, PA2295, Boster), anti-CD68 antibody (1:200 dilution, 28058–1-AP, Proteintech), anti-IBA1 antibody (1:100 dilution, CY7217, Abways), or anti-ST2 antibody (1:200 dilution, PRS3363, Sigma) at 4 °C overnight.

Techniques: Transformation Assay, Western Blot, Labeling, Immunofluorescence, Expressing

Journal: Frontiers in Neuroscience

Article Title: Subcommissural Organ-Spondin-Derived Peptide Restores Memory in a Mouse Model of Alzheimer’s Disease

doi: 10.3389/fnins.2021.651094

Figure Lengend Snippet: Early stage daily treatment with NX210 or NX210c reduces pathological hallmarks of Alzheimer’s disease. Mice were treated intraperitoneally once a day with vehicle, NX210 or NX210c (2 mg/kg), or orally with donepezil (DPZ, 1 mg/kg) one hour after intracerebroventricular injection of scrambled control peptides (Ctrl) or amyloid-beta 25 – 35 (Aβ 25 – 35 ) oligomers. They were sacrificed at day 11 (D11) and cerebral structures [hippocampi (A–D) and prefrontal cortices (E–H) ] were collected for biochemical analyses of levels of Aβ 1 – 42 ( A ; ELISA), phosphorylated-tau on threonine 181 [pTau; ( B ; ELISA)], tumor necrosis factor-α [TNFα; ( C ; ELISA)], lipid peroxidation [LPO; ( D; measure of cumene hydroperoxide (CHP) absorbance)], glial fibrillary acidic protein [GFAP; ( E ; ELISA)], caspase-12 [Casp-12; ( F ; ELISA)], synaptophysin [SYP; ( G ; ELISA)] and postsynaptic density protein 95 [PSD95; ( H ; ELISA)]. NX210 and NX210c decreased the levels of all those pathological hallmarks of AD and increased synaptogenesis. The data are expressed in CHP equivalents (CHPeq) per wet weight of tissue (D) or in pg per mg of tissue ( other items ) and presented as means and SEM. One-way ANOVA followed by Tukey’s multiple comparisons test: ### p < 0.001, # p < 0.05 compared with Ctrl; *** p < 0.001, ** p < 0.01 comp ared with Aβ 25 – 35 ; £££ p < 0.001, £ p < 0.05 compared with Aβ 25 – 35 + DPZ; $$$ p < 0.001 Aβ 25 – 35 + NX210 vs. Aβ 25 – 35 + NX210c, n = 6 Ctrl, n = 5 Aβ 25 – 35 , Aβ 25 – 35 + DPZ, Aβ 25 – 35 + NX210c, n = 4 Aβ 25 – 35 + NX210. These analyses do not include two outliers identified for caspase 12 ELISA using the Grubbs test (red circles) ( p < 0.05).

Article Snippet: Based on the manufacturers’ instructions, ELISA for mouse amyloid-beta 1 – 42 (Aβ 1 – 42 ; Cloud-Clone Corp., Houston, TX, United States), phosphorylated-tau on threonine 181 (pTau; Thermo Fisher, Waltham, MA, United States), tumor necrosis factor alpha (TNF-α; Thermo Fisher) (from left hippocampi), glial fibrillary acidic protein (GFAP; Cloud-Clone Corp.), caspase-12 (Cloud-Clone Corp.) (from left prefrontal cortices), synaptophysin (Cloud-Clone Corp.) and postsynaptic density protein 95 (PSD95; Cloud-Clone Corp.) (from right prefrontal cortices) were performed.

Techniques: Injection, Enzyme-linked Immunosorbent Assay